Journal: Molecular Diversity

Article Title: Subtractive sequence analysis aided druggable targets mining in Burkholderia cepacia complex and finding inhibitors through bioinformatics approach

doi: 10.1007/s11030-022-10584-5

Figure Lengend Snippet: Genome statistics of 19 BCC strains isolated from humans

Article Snippet: To improve the reliability of these proteins as potential broad-spectrum therapeutic targets, we compared the file of 37 BCC proteins with Burkholderia gladioli ATCC 10,248 (BLASTp, e-value ≤ 0.0001), another closely related BCC species.

Techniques: Isolation, Infection, Environmental Sampling, Aerosol

Journal: Molecular Diversity

Article Title: Subtractive sequence analysis aided druggable targets mining in Burkholderia cepacia complex and finding inhibitors through bioinformatics approach

doi: 10.1007/s11030-022-10584-5

Figure Lengend Snippet: Genome statistics of 7 BCC strains isolated from plants

Article Snippet: To improve the reliability of these proteins as potential broad-spectrum therapeutic targets, we compared the file of 37 BCC proteins with Burkholderia gladioli ATCC 10,248 (BLASTp, e-value ≤ 0.0001), another closely related BCC species.

Techniques: Isolation, Northern Blot

Journal: Molecular Diversity

Article Title: Subtractive sequence analysis aided druggable targets mining in Burkholderia cepacia complex and finding inhibitors through bioinformatics approach

doi: 10.1007/s11030-022-10584-5

Figure Lengend Snippet: Genome statistics of 21 BCC strains isolated from environmental sources

Article Snippet: To improve the reliability of these proteins as potential broad-spectrum therapeutic targets, we compared the file of 37 BCC proteins with Burkholderia gladioli ATCC 10,248 (BLASTp, e-value ≤ 0.0001), another closely related BCC species.

Techniques: Isolation, Aerosol, Northern Blot

Journal: Glycobiology

Article Title: Cryptococcus neoformans UGT1 encodes a UDP-Galactose/UDP-GalNAc transporter

doi: 10.1093/glycob/cww078

Figure Lengend Snippet: C. neoformans strains used in these studies

Article Snippet: UGT1 was identified by BLASTP searches against C. neoformans predicted proteins (Broad Institute; C. neoformans var. grubii H99 database) using known UDP-galactose transporters from Schizosaccharomyces pombe ( {"type":"entrez-protein","attrs":{"text":"NP_588041","term_id":"19075541","term_text":"NP_588041"}} NP_588041 ), Arabidopsis thaliana ( {"type":"entrez-protein","attrs":{"text":"NP_565158.1","term_id":"18411611","term_text":"NP_565158.1"}} NP_565158.1 ), Caenorhabditis elegans ( {"type":"entrez-protein","attrs":{"text":"NP_001255676.1","term_id":"392901327","term_text":"NP_001255676.1"}} NP_001255676.1 ) and Homo sapiens ( {"type":"entrez-nucleotide","attrs":{"text":"NC_000023.11","term_id":"568815575","term_text":"NC_000023.11"}} NC_000023.11 ).

Techniques:

Journal: Glycobiology

Article Title: Cryptococcus neoformans UGT1 encodes a UDP-Galactose/UDP-GalNAc transporter

doi: 10.1093/glycob/cww078

Figure Lengend Snippet: Topology of C. neoformans Ugt1 as predicted by TMHMM server v 2.0, showing 10 putative transmembrane domains and long N- and C-terminal cytosolic tails. Arrowheads indicate the new N-terminus for each N-terminal truncation and the terminal residue of the single C-terminal truncation (C1); see text and Table III for details.

Article Snippet: UGT1 was identified by BLASTP searches against C. neoformans predicted proteins (Broad Institute; C. neoformans var. grubii H99 database) using known UDP-galactose transporters from Schizosaccharomyces pombe ( {"type":"entrez-protein","attrs":{"text":"NP_588041","term_id":"19075541","term_text":"NP_588041"}} NP_588041 ), Arabidopsis thaliana ( {"type":"entrez-protein","attrs":{"text":"NP_565158.1","term_id":"18411611","term_text":"NP_565158.1"}} NP_565158.1 ), Caenorhabditis elegans ( {"type":"entrez-protein","attrs":{"text":"NP_001255676.1","term_id":"392901327","term_text":"NP_001255676.1"}} NP_001255676.1 ) and Homo sapiens ( {"type":"entrez-nucleotide","attrs":{"text":"NC_000023.11","term_id":"568815575","term_text":"NC_000023.11"}} NC_000023.11 ).

Techniques: Residue

Journal: Glycobiology

Article Title: Cryptococcus neoformans UGT1 encodes a UDP-Galactose/UDP-GalNAc transporter

doi: 10.1093/glycob/cww078

Figure Lengend Snippet: Cells lacking Ugt1 are more efficiently phagocytosed and killed by THP-1 cells than wild-type C. neoformans. (A) Phagocytic index (engulfed fungi/100 host cells) of strains grown in YPD (−/+ opsonization) or in inducing media (+ opsonization). (B) Survival of YPD-grown, opsonized fungi after internalization by THP-1 cells. Data are representative of three independent experiments performed with n = 3 (*, P < 0.01; **, P < 0.001).

Article Snippet: UGT1 was identified by BLASTP searches against C. neoformans predicted proteins (Broad Institute; C. neoformans var. grubii H99 database) using known UDP-galactose transporters from Schizosaccharomyces pombe ( {"type":"entrez-protein","attrs":{"text":"NP_588041","term_id":"19075541","term_text":"NP_588041"}} NP_588041 ), Arabidopsis thaliana ( {"type":"entrez-protein","attrs":{"text":"NP_565158.1","term_id":"18411611","term_text":"NP_565158.1"}} NP_565158.1 ), Caenorhabditis elegans ( {"type":"entrez-protein","attrs":{"text":"NP_001255676.1","term_id":"392901327","term_text":"NP_001255676.1"}} NP_001255676.1 ) and Homo sapiens ( {"type":"entrez-nucleotide","attrs":{"text":"NC_000023.11","term_id":"568815575","term_text":"NC_000023.11"}} NC_000023.11 ).

Techniques: